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产品目录

CD45/PTPRC抗体图片
产品货号:
Z31422
中文名称:
CD45/PTPRC抗体
英文名称:
Anti-CD45/PTPRC Antibody (Clone#2H3)
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称Anti-CD45/PTPRC Antibody (Clone#2H3)
指标别名B220;CD45;CD45R;GP180;L CA;LCA;Leukocyte common antigen;LY5;PTPRC;RPTPC;T200
克隆性Monoclonal
检验物种human
应用范围WB,IHC,IF,FCM
基因名称PTPRC
克隆号2H3
抗体来源Mouse
抗体类型IgG2b
免疫原A synthetic peptide corresponding to a sequence at the C-terminus of human CD45(1214-1254aa EQYQFLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVDKVK),different from the related mouse sequence by eight amino acids,and from the related rat sequence by ten amino acids.
实际分子量180~250KD
成分500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol.
纯化方式protein G purified.
浓度500μg/mL
产品形态溶液
保存条件12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料CD45 (Cluster of Differentiation 45),also known as PTPRC,LCA or CD45R,is an enzyme that,in humans,is encoded by the PTPRC gene.It is a member of the protein tyrosine phosphatase (PTP) family.CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1.This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP).Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins.In vitro,CD45 directly dephosphorylates and binds to JAKs.Functionally,CD45 negatively regulates interleukin-3-mediated cellular proliferation,erythropoietin-dependent hematopoiesis,and antiviral responses in vitro and in vivo.In addition,CD45 has been best studied in T cells,where it determines T cell receptor signaling thresholds.CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton.Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Uniprot IDP08575
推荐配套试剂Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P).
基因名全称protein tyrosine phosphatase,receptor type,C
蛋白名全称Receptor-type tyrosine-protein phosphatase C
推荐稀释比
Western blot(WB) 1:500~2000
Immunohistochemistry in paraffin section (IHC) 1:50~400
Immunofluorescence (IF) 1:50~400
Flow cytometry (FCM) 1~3μg/1×106 cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

相关搜索:CD45/PTPRC抗体CD45抗体PTPRC单抗Anti-CD45/PTPRC Antibody (Clone#2H3)
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