产品货号：
Z31422
中文名称：
CD45/PTPRC抗体
英文名称：
Anti-CD45/PTPRC Antibody (Clone#2H3)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-CD45/PTPRC Antibody (Clone#2H3) |
| 指标别名 | B220;CD45;CD45R;GP180;L CA;LCA;Leukocyte common antigen;LY5;PTPRC;RPTPC;T200 |
| 克隆性 | Monoclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,IF,FCM |
| 基因名称 | PTPRC |
| 克隆号 | 2H3 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2b |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human CD45(1214-1254aa EQYQFLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVDKVK),different from the related mouse sequence by eight amino acids,and from the related rat sequence by ten amino acids. |
| 实际分子量 | 180～250KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | CD45 (Cluster of Differentiation 45),also known as PTPRC,LCA or CD45R,is an enzyme that,in humans,is encoded by the PTPRC gene.It is a member of the protein tyrosine phosphatase (PTP) family.CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1.This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP).Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins.In vitro,CD45 directly dephosphorylates and binds to JAKs.Functionally,CD45 negatively regulates interleukin-3-mediated cellular proliferation,erythropoietin-dependent hematopoiesis,and antiviral responses in vitro and in vivo.In addition,CD45 has been best studied in T cells,where it determines T cell receptor signaling thresholds.CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton.Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates. |
| Uniprot ID | P08575 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P). |
| 基因名全称 | protein tyrosine phosphatase,receptor type,C |
| 蛋白名全称 | Receptor-type tyrosine-protein phosphatase C |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunofluorescence (IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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